tcid 50 assay  (GraphPad Software Inc)

Journal: Vaccines

Article Title: Quadrivalent Formulation of Intranasal Influenza Vaccine M2SR (M2-Deficient Single Replication) Protects against Drifted Influenza A and B Virus Challenge

doi: 10.3390/vaccines11040798

Figure Lengend Snippet: M2SR Vaccine Candidates for Ferret Study.

Article Snippet: Virus titers in nasal washes and organ homogenates were determined by a TCID 50 assay in MDCK cells (Sigma-Aldrich, St. Louis, MO, USA, Cat#84121903), as previously described [ ].

Techniques:

Journal: Vaccines

Article Title: Quadrivalent Formulation of Intranasal Influenza Vaccine M2SR (M2-Deficient Single Replication) Protects against Drifted Influenza A and B Virus Challenge

doi: 10.3390/vaccines11040798

Figure Lengend Snippet: Nasal wash titers from M2SR-vaccinated ferrets following the challenge with the H1N1 influenza virus. Nasal washes were collected on days 1, 3, and 5 post-challenge. Virus loads in samples were determined by a TCID 50 assay in MDCK cells. Open faced symbols (e.g., triangles, diamonds, circles, etc.) represent virus titers from individual mice within the group, while colored bars represent the mean titer for the indicated group. The detection limit of the assay was 1.5 log 10 TCID 50 /mL, as indicated by a horizontal dotted line. Solid symbols above horizontal black bars (e.g., *, #) indicate results that were significantly different from those of the PBS-Mock group: * p < 0.05, ** p < 0.005, and # p < 0.0005, as compared by an unpaired t test.

Article Snippet: Virus titers in nasal washes and organ homogenates were determined by a TCID 50 assay in MDCK cells (Sigma-Aldrich, St. Louis, MO, USA, Cat#84121903), as previously described [ ].

Techniques:

Journal: Vaccines

Article Title: Quadrivalent Formulation of Intranasal Influenza Vaccine M2SR (M2-Deficient Single Replication) Protects against Drifted Influenza A and B Virus Challenge

doi: 10.3390/vaccines11040798

Figure Lengend Snippet: Organ titers from M2SR vaccinated ferrets following a challenge with the H1N1 influenza virus. Nasal turbinates ( A ), trachea ( B ), and lung ( C ) were collected from 4 ferrets per group on day 3 post-challenge. Virus loads in samples were determined by a TCID 50 assay in MDCK cells. Open faced symbols (e.g., triangles, diamonds, circles, etc.) represent virus titers from individual mice within the group, while colored bars represent the mean titer for the indicated group. The detection limit of the assay was 1.5 log 10 TCID 50 /mL, as indicated by a horizontal dotted line.

Article Snippet: Virus titers in nasal washes and organ homogenates were determined by a TCID 50 assay in MDCK cells (Sigma-Aldrich, St. Louis, MO, USA, Cat#84121903), as previously described [ ].

Techniques: